MAHESH SHARMA

ADDRESS:
  • A133 A New Ashok Nagar New Dehli-110096
  • Barnagar, Dist- Ujjain, (M.P.) (INDIA)
CONTACTS:
  • E-mail:drsharmamahesh@gmail.com
  • Mobile: : +91-9630-800-789, Office: : +91-11-470-742-63

OBJECTIVE

    To be a part of professional organization where I could apply and hone my acquired skills, to work to my full potential and ability in a challenging and intellectually stimulating environment in the field of analytical and forensic chemistry.

EDUCATIONAL QUALIFICATION

  • M.Sc, 2012 (Forensic science), Anglia Ruskin University, Cambridge, England (UK).
  • M.Sc, 2008 (Bioinformatics), Sikkim Manipal University.
  • B.Sc, 2005 (Microbiology + Pharma chemistry), Maharaja Ranjit Singh College. DAVV – INDORE
  • 12th , 2001 from SVH School – INDORE (M.P. Board)
  • 10th ,1998 from Govt. H school – Ujjain (M.P. Board)

WORK EXPERIENCE

  • Working as a Forensic Expert & Investigator at Sherlock Institution of Forensic Science India (SIFS INDIA) from 1-May-2013 to date
  • Working as a Forensic Expert & Investigator at Sparkle Institution of Forensic Science India from 1-Oct-2012 to 30-April-2013.

PUBLICATIONS, MAMBERSHIP AND PROFESSIONAL QUALIFICATION:

  • Sharma M. (2013) Pharmacophore Modeling for leukemia. International Science Congress Association (ISCA) International E- Publication: Pharmaceutical Sciences: Master, pp 1-93. ISBN: 978-93-84648-60-2
  • Sharma, M. (2013).  Method development for analysis of Amphetamine-type drugs using GC-MS Forensic Toxicology & drugs Abuse. Saarbrücken, Germany: LAP Lambert Academic Publishing. ISBN-13: 978-3-659-45267-3
  • Sharma, M. (2013). Abstract: Method development for analysis of Amphetamine-type stimulants using GC-MS. Indore-INDIA: International Science Congress Association (ISCA) souvenir. -ISC-2012-9FS-06, PN: 165.
  • Sharma M. (2015). Molecular Modeling against Leukemia. International Science Congress Association (ISCA) International E- Publication: Biological Sciences Research, pp 1-337. ISBN 978-93-84648-61-9
  • Sharma M. and Singh R.K. (2015). Critical Evaluation and Contribution of Virtopsy to Solved Crime. Research Journal of Forensic Sciences: 3 (1) 1-9.
  • Sharma M. and Singh R.K.  (2015). Evaluation of Criminal Investigation with Time & New Technology. Research Journal of Forensic Sciences: 3 (2) 6-9.
  • Sharma M. and Singh R.K. (2015). Extraction, analysis and quantization of endogenous level of GHB from Human Hair. Research Journal of Forensic Sciences: 3 (3) 1-6.
  • Dr Singh R.K, Dr. Singh N., Ms. Khanna S., Ms. Mishra N., Sharma M.  (04/2015). Conference Paper: Forensic Psychological perspective in sexual assault cases. National Conference on "Role of Forensic Science in Crime against Women", Cancer Hospital, Gwalior.
  • Dr Singh R.K., Dr. Singh N, Sharma M., Sharma S., Ms. Mishra N.  (04/2015). Conference Poster: Forensic Analysis in Violence against women. National Conference on "Role of Forensic Science in Crime against Women", Cancer Hospital, Gwalior.
  • Khanna S., Sharma M. and Singh R.K.  (2015). Cheiloscopy: Frequency of Pattern in different Quadrant in females. Research Journal of Forensic Sciences: 3 (4) 1-9.
  • Dr Singh R.K., Yan Peng and Sharma Mahesh. (2015). Human hair analysis and quantitation of endogenous level of Gamma Hydroxybutyric Acid (GHB). Journal of Forensic, Toxicology and Medicolegal Analysis: 1 (1): 3-5.
  • Dr Singh R.K., Sharma, M. & Wepeba Pet-Paul (2015). Abstract: A case of three Deltas in a Fingerprint. International Science Congress Association (ISCA) INDIA, At Tribhuvan University, Kathmandu, Nepal, souvenir. - ISCA-ISC-2015-9FMDN-20-Oral, PN: 116.
  • Afreen Tarannum, Dr. Ranjeet Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: Innovative Approach for Managing Crime Scene by First Responder. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Deepika Singh, Dr. Ranjeet Kr. Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: Use of DNA Technology in Forensic Dentistry. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Garima Rastogi, Dr. Ranjeet Kr. Singh, Dr. Shefali Gola, Mr. Mahesh Sharma (2016). Conference Paper Abstract: time dependent quantification of dna from bloodstains present on different fabrics. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Kaneeka Joshi, Dr. Ranjeet Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: Overlapped fingerprint Identification using MATLAB Software. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Kratika Mishra, Dr. Ranjeet Kr. Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: Diversity of Diatom Species from Two Tributaries of Betwa River: A Comparative Approach. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Nirupama Gourh, Dr. Ranjeet Kr. Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: Fingerprints and their rustle in investigating and gathering it from crime scene. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Pragati Mishra, Dr. Ranjeet Kr. Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: Human Hair Analysis among Different Castes Having Potential Application in Forensic Investigation. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow
  • Ms. Sonia Kumari, Dr. Ranjeet Kr. Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: sensitivity of kastle meyer’s test. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Vivek khare, Dr. Ranjeet Kr. Singh, Dr. Ankit Srivastava, Mr. Mahesh Sharma (2016). Conference Paper Abstract: particle size distribution of soil samples of various places. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
  • Yamini Tanwar, Dr. Ranjeet Kr. Singh, Mr. Mahesh Sharma (2016). Conference Paper Abstract: analysis of bite marks at crime scene. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.

    Fellow Member of SIFS INDIA.

    Fellow Member of International Science Congress Association (ISCA).

    DOEACC “A” level (PG-Level) in Bioinformatics Delhi. (A unit of DOEACC Society,  An autonomous body  of Dept. of Information  Technology, Ministry of Communications & Information Technology, Govt. of India) one paper left.

SUBJECTS STUDIED

    Forensic science

    Evidence Collection and Management (30 credits), Mastering Forensic Evidence (30 credits), Mastering Forensic Analysis (30 credits), Specialised Topics (15 credits), Research Methods and Preparation (15 credits), Masters Research Project (60 credits) These are mostly a combination of laboratory and court reports (including witness statements), oral presentations and closed examinations. Some essay and report writing was required. The research project is by submission of a dissertation (20000 words) and oral presentation.

    Experimental Forensic

    Fingerprints analysis, hair analysis, drugs analysis, paint analysis, fibre analysis, documents analysis, foot prints, body fluids, blood pattern analysis using microbial, spectroscopic and mechanical technique such as, FTIR, GC-MS comparison microscope, low and high power microscope. In addition of crime scene management, in a group we visit crime scene, collect evidence then this evidences was analyzed in laboratory and finally witness statement was presented in moot court.

    Bioinformatics:

    Data Base for Bioinformatics & Computing, Biological System & Bioinformatics, Windows Application, MS-Office, HTML, Multimedia, Static & Probability, Computational Method for Sequence Analysis, Biological Data Bank & Analysis, (OOPS), Perl for bioinformatics, VB, Drug Design &Gene Therapy, Plant Genomics, JAVA, Photoshop, Flash, Dream Weaver, Data Warehousing & Data Mining, Numerical Method & Optimization Tech, Emerging areas in Bioinformatics.

    Pharma Chemistry:

    TLC, HPLC, GLC, paper chromatography & column chromatography. Drugs preparation: - syrup, lotion, Drops crystallization. Drugs activity, QSAR, MM, ADME, IP, BP, Body Receptor, Na, K, Mg,-Channels, Chemical Isomerism.

    Microbiology:

    Soil Microbiology, Food Microbiology, Water Microbiology, Waste Water Treatment, Air Microbiology, Fundamentals of Genetics, DNA Replication and Gene Structure, Mutation, Genetic Recombination, Infection, Immune System , Immune Response, Antigens and Antibodies, Antigen and Antibody Reactions, Fundamentals of Industrial Microbiology, Fermentor Design, Scale up, Industrial production, Bioassays, Quality Cont, Colorimetry and Spectrophotometry, Separation Techniques, Microorganisms in Agriculture, Geomicrobiology, Pharmaceutical, Biotechnology, Food from Microbes, Advanced Microbiology, Regulation of Gene Activity, Genetic Engineering, Gene Cloning, Techniques in Molecular Biology, Applications and Biohazards of Genetic Engineering

    Experimental Microbiology:

    Isolation of microorganisms by Sector Plate/Pour plate method, Differential staining, Special staining, Isolation of microorganisms from water / sewage / food / curd / canker/soil, Qualitative estimation of carbohydrates / proteins / lipids, Total count of RBC/WBC/Differential count of WBC/Hb estimation, Antigen-antibody reactions – Widal /VDRL, Isolation of mutants by replica plating technique/gradient plate technique, Identification of medically important organisms Staphylococcus / Streptococcus, E.coli , Urine analysis / Antibiotic sensitivity testing / Gradient Plate Technique, Qualitative and Quantitative analysis of water/food/milk/sewage, Microbial assay of Antibiotics/ Isolation of industrially important microbes/Paper Chromatography/TLC, Isolation of bacterial/fungal DNA, Quantitative estimation of DNA/RNA, Isolation of Rhizobium .

PROJECT & WORKSHOP

  • M.Sc. Forensic. Project: Method development for analysis of Amphetamine types stimulants using GC-MS
  • Abstract: A gas chromatography–mass spectrometry (GC–MS) method was developed and validated for the concurrent requirement and quantification of d-amphetamine sulphate, methamphetamine (MA), Ibuprofen, Piperazine hexahydrate, 1-(4-methoxyphenyl) piperazine (pMeOPP), 1-(4-Trifluoromethylphenyl) piperazine (4-TFMPP), 1-Benzylpiperazine (BZP) and 1-(2- methoxyphenyl) piperazine (oMeOPP), using Quinoline as an internal standard. All this abused drugs was purchased by chemical supplier. Different dilution preparation was calculated then specific amount of volume was used for different methods. After solutions were evaporated to dryness, they were derivatized using 50, 100, 150 and 200 µL pentafluoropropionic acid anhydride (PFPA) at 80oC for 15, 30, 45 and 60 minutes. Time optimisation and derivatisation optimisation give very good results at 150 µL volume of PFPA with 80oC temperature and 30 minutes duration of heating time. In addition as a second derivatisaing agent N,O-bis(trimethylsilyl)-trifluoroacetamide and trimethylchlorosilane (BSTFA:TMCS) were used at 70oC for 30 minutes and analyzed by GC–MS. The linear range was 0.025–0.5 mg/mL for all drugs, with the coefficients of determination (R2). The limits of detections (LODs) and the limits of quantifications (LOQs) for each analyte were lower than 0.0111 mg/mL and 0.0317 mg/mL. The method was proved and is also suitable for the simultaneous detection and quantification of all selected drugs.

  • Project at BIOMED informatics, drug discovery and clinical research center, medwin hospitals in Hyderabad: Molecular Targeting against leukaemia.
  • Abstract: Chromic myelogenus leukaemia (CML) is characterized by the Philadelphia (Ph) chromosome and bcr/abl gene rearrangement which occurs in pluripotent hematopoietic progenitor cells expressing the c-kit receptor tyrosine kinase (KIT). Alternative splicing is a crucial mechanism for generating protein diversity. Different splice variants of a given protein can display different and even antagonistic biological functions. Therefore, appropriate control of their synthesis is required to assure the complex orchestration of cellular processes within multicellular organisms. One of the most exciting developments in cancer research in recent years has been the clinical validation of molecularly targeted drugs that inhibit the action fo pathogenic tyrosine kinases. The clinical validation of these “first-generation” tyrosine kinase inhibitors has been the prelude to a second wave of advances in molecular targeting that is expected to further change the way we classify and treat cancer. Efforts are now being directed at identifying the tumor subtypes and patients who will benefit the most from the drugs. Agents directed against new molecular targets are also being explored. AIMs: Homology modeling of tyrosine kinase and Screening of compound library for tyrosine kinase inhibitors

  • M.Sc. Bioinformatics Project: Pharmacophore Modeling in LEUKEMIA Disease
  • Abstract: Three genetically linked leukemic genes are selected from the chromosomes (ABL1 chro-9, AFF1 chro-4, MKL1 chro-22). From these genes we find out some interest Protein having more than four ligands, For Leukemia disease a best suitable Pharmacophore Model was prepared with the help of Ligandscout 1.03 Tool. If any drug can bind with this Pharmacophore then we can have 100% treatment of genetically linked LEUKEMIA. In this whole project study, we have refered to NCBI, TIGR, EBI, HPRD, and SWISS-PROT for data base.

  • B.Sc. Project: Design of Fully Working Fermenter Model:
  • In this 2 litre fermenter model we can incubate Organism, add antifoaming agent, temperature control and have a couple of impeller to rotate. It uses 220 volt power supply. In a final Project of B.Sc. Microbiology.

  • Attended workshop on “Biological sequences analysis and application in crop improvement” at G.B.Pant University (Uttarakhand) Nov16-17,2007
  • Developed an Offline Web site For DOEACC final Project.
  • Six month DOTNET training at Alien SoftNet Technologies Pvt. Ltd.

TECHNICAL SKILLS

Forensic tools GC-MS, FTIR, MSP including all microscopic tech.
Bioinformatics tools: BLAST, FASTA, Ligandscout 1.03, T-COFFEE, CLUSTAL-W, cn3d, Geneious 2.5.2
Databases: NCBI, TIGR, EBI, HPRD, ENTREZ, EMBL, GENBANK, SWISS-PROT, ICTV MetaCyc, EcoCyc, KEGG.
Language: DOTNET, C, C++, VB, PERL, Core Java, HTML and XML
OS: Window 98, 2000, XP, 7. Linux
Database: SQL- server 2000, SQL- server 2005

AREA OF INTEREST

    My area of interest is broad during my education, whatever I learned I applied in my work according to my needs. But I focused on Crime scene investigation, Fingerprint analysis, hair analysis, drugs analysis, paint analysis, fibre analysis, document analysis, foot print and blood pattern analysis. Software developer in DOTNET Technology. Computer Aided Drug Designing, Molecular Modelling, Computational Biology, Immunology, Recombinant DNA Technology, Genomics & Proteomics, Metabolic pathways, Gene Sequence Analysis, Protein Sequence Analysis, Discovery of genuine drugs for Cancer using Bioinformatics Tools, Phylogenetic Analysis, Virtual Screening and Docking.

PERSONAL PARTICULARS

NAME Mahesh Sharma
DATE OF BIRTH: 24-01-1984
FATHER’S NAME Satyanarayan ji Sharma
MOTHER’S NAME: Laleeta ji Sharma
NATIONALITY: Indian
GENDER: Male
MARITAL STATUS: Single
LANGUAGES KNOWN: English, Hindi
HOBBIES: Net Browsing, Traveling and making friends.
PERMANENT ADDRESS: 191- Sharma Niwas Junashar BARNAGAR , Dist- Ujjain (M.P.) (INDIA)
PHONE NO: +91-9630-800-789
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