MAHESH SHARMA

OBJECTIVE

To be a part of professional organization where I could apply and hone my acquired skills, to work to my full potential and ability in a challenging and intellectually stimulating environment in the field of analytical and forensic chemistry.

EDUCATIONAL QUALIFICATION

• M.Sc, 2012 (Forensic science), Anglia Ruskin University, Cambridge, England (UK).
• M.Sc, 2008 (Bioinformatics), Sikkim Manipal University.
• B.Sc, 2005 (Microbiology + Pharma chemistry), Maharaja Ranjit Singh College. DAVV – INDORE
• 12th , 2001 from SVH School – INDORE (M.P. Board)
• 10th ,1998 from Govt. H school – Ujjain (M.P. Board)

WORK EXPERIENCE

• Deployed as First Responder by Kenyon International Emergency Services. An Air India Express Boeing overran the runway while landing in monsoon rains at Kozhikode Airport. The aircraft was part of the Vande Bharat Mission, repatriating Indian nationals from Dubai, UAE. I was activated to recover and repatriate the personal effects at the incident site. 10-Aug-2020 to 23-Aug-2020
• Associates & Freelance Working as a Forensic Expert & Investigator at Sherlock Institution of Forensic Science India (SIFS INDIA) from 1-May-2013 to date
• Worked as a Forensic Expert & Investigator at Sparkle Institution of Forensic Science India from 1-Oct-2012 to 30-April-2013.
• Working as Kenyon International Emergency Services Team Member Regional Coordinator INDIA & Associates Mahesh TMRC | Kenyon ALL TMRC
• Special Assistance Team Member & Go Team Member of Kenyon International Emergency Services.

PUBLICATIONS:

• Books

  • Sharma M. (2013) Pharmacophore Modeling for leukemia. International Science Congress Association (ISCA) International E- Publication: Pharmaceutical Sciences: Master, pp 1-93. ISBN: 978-93-84648-60-2
  • Sharma, M. (2013). Method development for analysis of Amphetamine-type drugs using GC-MS Forensic Toxicology & drugs Abuse. Saarbrücken, Germany: LAP Lambert Academic Publishing. ISBN-13: 978-3-659-45267-3 Amazon | Flipkart
  • Sharma M. (2015). Molecular Modeling against Leukemia. International Science Congress Association (ISCA) International E- Publication: Biological Sciences Research, pp 1-337. ISBN 978-93-84648-61-9

• Journals Research Paper

• 1. Sharma M. and Singh R.K. (2015). Critical Evaluation and Contribution of Virtopsy to Solved Crime. Research Journal of Forensic Sciences: 3 (1) 1-9.
• 2. Sharma M. and Singh R.K. (2015). Evaluation of Criminal Investigation with Time & New Technology. Research Journal of Forensic Sciences: 3 (2) 6-9.
• 3. Sharma M. and Singh R.K. (2015). Extraction, analysis and quantization of endogenous level of GHB from Human Hair. Research Journal of Forensic Sciences: 3 (3) 1-6.
• 4. Khanna S., Sharma M. and Singh R.K. (2015). Cheiloscopy: Frequency of Pattern in different Quadrant in females. Research Journal of Forensic Sciences: 3 (4) 1-9.
• 5. Singh R.K., Yan Peng and Sharma Mahesh. (2015). Human hair analysis and quantitation of endogenous level of Gamma Hydroxybutyric Acid (GHB). Journal of Forensic, Toxicology and Medicolegal Analysis: 1 (1): 3-5.
• 6. Singh R.K., Sharma, M., Tarannum A., Wepeba Pet-Paul & Leddy B. (2018). A case of three Deltas in a Fingerprint. Journal of Forensic Research. 2018 March-28;9 (2): 1-4.
• 7. Kwaku Mark Asiedu, Sharma Mahesh and Singh Ranjeet Kumar (2018) Estimation of Age from Teeth, Academic Journal of Anthropological Studies - 1 (2):6-10.
• 8. Singh Ranjeet Kumar, Sharma Mahesh and Azeen Sheza (2018) Estimation of Body Mass from Skeletal Remains, Academic Journal of Anthropological Studies - 1 (2):16-19.
• 9. Hashakimana Clare, Singh Ranjeet Kumar, Sharma Mahesh and Tarannum Afreen (2019) Comparative Study of Original, Photocopy and Colored Copy of Handwritten Documents Using a Stereo Microscope, Academic Journal of Forensic Sciences - 2 (1):1-7.
• 10. Shukla Jaya, Shrivastava Ankit, Sharma Mahesh and Singh Ranjeet Kumar (2019) Comparative Study of Handwriting among English Medium and Hindi Medium Students, Academic Journal of Forensic Sciences - 2 (1):8-17.

• Paper Presentation

• 1. Sharma, M. (2013). Abstract: Method development for analysis of Amphetamine-type stimulants using GC-MS. 2nd International Science Congress Association (ISCA) at Vrindavan-INDIA souvenir. -ISC-2012-9FS-06, PN: 165.
• 2. Singh R.K, Singh N., Khanna S., Mishra N., Sharma M. (04/2015). Abstract: Forensic Psychological perspective in sexual assault cases. National Conference on "Role of Forensic Science in Crime against Women", Cancer Hospital, Gwalior.
• 3. Singh R.K., Sharma, M. & Wepeba Pet-Paul (2015). Abstract: A case of three Deltas in a Fingerprint. 3rd International Science Congress Association (ISCA) INDIA, At Tribhuvan University, Kathmandu, Nepal, souvenir. - ISCA-ISC-2015-9FMDN-20-Oral, PN: 116.
• 4. Tarannum Afreen, Singh Ranjeet, Sharma Mahesh (2016). Conference Paper Abstract: Innovative Approach for Managing Crime Scene by First Responder. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 5. Singh Deepika, Singh Ranjeet, Sharma Mahesh (2016). Conference Paper Abstract: Use of DNA Technology in Forensic Dentistry. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 6. Rastogi Garima, Singh Ranjeet Kr, Gola Shefali, Sharma Mahesh (2016). Conference Paper Abstract: time dependent quantification of dna from bloodstains present on different fabrics. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 7. Joshi Kaneeka, Singh Ranjeet, Sharma Mahesh (2016). Conference Paper Abstract: Overlapped fingerprint Identification using MATLAB Software. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 8. Mishra Kratika, Singh Ranjeet Kr., Sharma Mahesh (2016). Conference Paper Abstract: Diversity of Diatom Species from Two Tributaries of Betwa River: A Comparative Approach. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 9. Gourh Nirupama, Singh Ranjeet Kr., Sharma Mahesh (2016). Conference Paper Abstract: Fingerprints and their rustle in investigating and gathering it from crime scene. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 10. Mishra Pragati, Singh Ranjeet Kr., Sharma Mahesh (2016). Conference Paper Abstract: Human Hair Analysis among Different Castes Having Potential Application in Forensic Investigation. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow
• 11. Kumari Sonia, Singh Ranjeet Kr., Sharma Mahesh (2016). Conference Paper Abstract: sensitivity of kastle meyer’s test. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 12. khare Vivek, Singh Ranjeet Kr., Srivastava Ankit, Sharma Mahesh (2016). Conference Paper Abstract: particle size distribution of soil samples of various places. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.
• 13. Tanwar Yamini, Singh Ranjeet Kr., Sharma Mahesh (2016). Conference Paper Abstract: analysis of bite marks at crime scene. Indian Congress of Forensic Medicine and Toxicology (ICFMT), Lucknow.

• Poster Presentation

• 1. Kumari Mohini, Azeen Sheza, Sharma Mahesh, Singh Ranjeet Kumar (04/2015) “Medical Infringement of primates leading to their extinction, A Forensic Scrutiny- Do Primates owe Us their Life?”, 3rd National Conference on “Forensic Science and Criminalistics.
• 2. Singh R.K., Singh N, Sharma M., Sharma S., Ms. Mishra N. (04/2015). Conference Poster: Forensic Analysis in Violence against women. National Conference on "Role of Forensic Science in Crime against Women", Cancer Hospital, Gwalior.

CONFERENCES/ SEMINARS/ WORKSHOPS/ MEMBERSHIP:

• 1. 2019 National Seminar on Roll of Science and Technnology "In Crime Prevention", Sponsored by B.T. Institute of Excellence & Indian Science Congress Association- Sagar Chapter in Collaboration With (The state Forensic Science Laboratory, Sagar) (8th-9th March, 2019)
• 2. 2019 International Seminar on Forensic Comparative Science: Generalizing Across Disciplines, Sanskriti University, Mathura (11th February, 2019)
• 3. FORENSIS AGORA 2018”, Galgotias University (21st -22nd April, 2018)
• 4. 2018 National Seminar on “Forensic with Law is never a Flaw”, Clue4Evidence and Maharaja’s College, Mysore University (09th – 10th March, 2018)
• 5. 2018 International Conference on “Advances in Forensic DNA Technology ICAFDT 2018” Bundelkhand University, Uttar Pradesh (22nd -24th February 2018)
• 6. Core Member in 7th International Science Congress (ISC-2017) on 8th and 9th December 2017 at College of Science and Technology, Rinchending, Phuentsholing, Chukkha, Bhutan with focal theme as " Widespread Research: Strengthening Nations and Spreading Happiness".
• 7. 2016 International Conference of Indian Congress of Forensic Medicine and Toxicology (ICFMT 2016), Lucknow, (18th -19th November 2016).
• 8. 2014 International Workshop on Forensic Sciences, National PG College, Lucknow University, Uttar Pradesh (13th September 2014)
• 9. Attended workshop on “Biological sequences analysis and application in crop improvement” at G.B.Pant University (Uttarakhand) Nov16-17,2007
• 10. Fellow Member of Sherlock Institution of Forensic Science India (SIFS INDIA).
• 11. 2016- Present Publication Head, International Association of Scientists and Researchers, Delhi India
• 12. 2017- Present Editor and Reviewer of the Academic Journal of Forensic Sciences
• 13. Fellow & Life Member “International Association of Scientists and Researchers” (IASR).
• 14. Fellow & Life Member Member of International Science Congress Association (ISCA).
• 15. Kenyon Regional Team Member Coordinator INDIA
• 16. Advance Certification Course in Graphology and Handwriting Analysis Institution: National Centre for Handwriting Studies, Pune India
• 17. DOEACC “A” level (PG-Level) in Bioinformatics Delhi. (A unit of DOEACC Society, An autonomous body of Dept. of Information Technology, Ministry of Communications & Information Technology, Govt. of India) one paper left.

SUBJECTS STUDIED

Forensic science

Evidence Collection and Management (30 credits), Mastering Forensic Evidence (30 credits), Mastering Forensic Analysis (30 credits), Specialised Topics (15 credits), Research Methods and Preparation (15 credits), Masters Research Project (60 credits) These are mostly a combination of laboratory and court reports (including witness statements), oral presentations and closed examinations. Some essay and report writing was required. The research project is by submission of a dissertation (20000 words) and oral presentation.

Experimental Forensic

Fingerprints analysis, hair analysis, drugs analysis, paint analysis, fibre analysis, documents analysis, foot prints, body fluids, blood pattern analysis using microbial, spectroscopic and mechanical technique such as, FTIR, GC-MS comparison microscope, low and high power microscope. In addition of crime scene management, in a group we visit crime scene, collect evidence then this evidences was analyzed in laboratory and finally witness statement was presented in moot court.

Bioinformatics:

Data Base for Bioinformatics & Computing, Biological System & Bioinformatics, Windows Application, MS-Office, HTML, Multimedia, Static & Probability, Computational Method for Sequence Analysis, Biological Data Bank & Analysis, (OOPS), Perl for bioinformatics, VB, Drug Design &Gene Therapy, Plant Genomics, JAVA, Photoshop, Flash, Dream Weaver, Data Warehousing & Data Mining, Numerical Method & Optimization Tech, Emerging areas in Bioinformatics.

Pharma Chemistry:

TLC, HPLC, GLC, paper chromatography & column chromatography. Drugs preparation: - syrup, lotion, Drops crystallization. Drugs activity, QSAR, MM, ADME, IP, BP, Body Receptor, Na, K, Mg,-Channels, Chemical Isomerism.

Microbiology:

Soil Microbiology, Food Microbiology, Water Microbiology, Waste Water Treatment, Air Microbiology, Fundamentals of Genetics, DNA Replication and Gene Structure, Mutation, Genetic Recombination, Infection, Immune System , Immune Response, Antigens and Antibodies, Antigen and Antibody Reactions, Fundamentals of Industrial Microbiology, Fermentor Design, Scale up, Industrial production, Bioassays, Quality Cont, Colorimetry and Spectrophotometry, Separation Techniques, Microorganisms in Agriculture, Geomicrobiology, Pharmaceutical, Biotechnology, Food from Microbes, Advanced Microbiology, Regulation of Gene Activity, Genetic Engineering, Gene Cloning, Techniques in Molecular Biology, Applications and Biohazards of Genetic Engineering

Experimental Microbiology:

Isolation of microorganisms by Sector Plate/Pour plate method, Differential staining, Special staining, Isolation of microorganisms from water / sewage / food / curd / canker/soil, Qualitative estimation of carbohydrates / proteins / lipids, Total count of RBC/WBC/Differential count of WBC/Hb estimation, Antigen-antibody reactions – Widal /VDRL, Isolation of mutants by replica plating technique/gradient plate technique, Identification of medically important organisms Staphylococcus / Streptococcus, E.coli , Urine analysis / Antibiotic sensitivity testing / Gradient Plate Technique, Qualitative and Quantitative analysis of water/food/milk/sewage, Microbial assay of Antibiotics/ Isolation of industrially important microbes/Paper Chromatography/TLC, Isolation of bacterial/fungal DNA, Quantitative estimation of DNA/RNA, Isolation of Rhizobium .

PROJECT & WORKSHOP

• M.Sc. Forensic. Project: Method development for analysis of Amphetamine types stimulants using GC-MS

Abstract: A gas chromatography–mass spectrometry (GC–MS) method was developed and validated for the concurrent requirement and quantification of d-amphetamine sulphate, methamphetamine (MA), Ibuprofen, Piperazine hexahydrate, 1-(4-methoxyphenyl) piperazine (pMeOPP), 1-(4-Trifluoromethylphenyl) piperazine (4-TFMPP), 1-Benzylpiperazine (BZP) and 1-(2- methoxyphenyl) piperazine (oMeOPP), using Quinoline as an internal standard. All this abused drugs was purchased by chemical supplier. Different dilution preparation was calculated then specific amount of volume was used for different methods. After solutions were evaporated to dryness, they were derivatized using 50, 100, 150 and 200 µL pentafluoropropionic acid anhydride (PFPA) at 80oC for 15, 30, 45 and 60 minutes. Time optimisation and derivatisation optimisation give very good results at 150 µL volume of PFPA with 80oC temperature and 30 minutes duration of heating time. In addition as a second derivatisaing agent N,O-bis(trimethylsilyl)-trifluoroacetamide and trimethylchlorosilane (BSTFA:TMCS) were used at 70oC for 30 minutes and analyzed by GC–MS. The linear range was 0.025–0.5 mg/mL for all drugs, with the coefficients of determination (R2). The limits of detections (LODs) and the limits of quantifications (LOQs) for each analyte were lower than 0.0111 mg/mL and 0.0317 mg/mL. The method was proved and is also suitable for the simultaneous detection and quantification of all selected drugs.

• Project at BIOMED informatics, drug discovery and clinical research center, medwin hospitals in Hyderabad: Molecular Targeting against leukaemia.

Abstract: Chromic myelogenus leukaemia (CML) is characterized by the Philadelphia (Ph) chromosome and bcr/abl gene rearrangement which occurs in pluripotent hematopoietic progenitor cells expressing the c-kit receptor tyrosine kinase (KIT). Alternative splicing is a crucial mechanism for generating protein diversity. Different splice variants of a given protein can display different and even antagonistic biological functions. Therefore, appropriate control of their synthesis is required to assure the complex orchestration of cellular processes within multicellular organisms. One of the most exciting developments in cancer research in recent years has been the clinical validation of molecularly targeted drugs that inhibit the action fo pathogenic tyrosine kinases. The clinical validation of these “first-generation” tyrosine kinase inhibitors has been the prelude to a second wave of advances in molecular targeting that is expected to further change the way we classify and treat cancer. Efforts are now being directed at identifying the tumor subtypes and patients who will benefit the most from the drugs. Agents directed against new molecular targets are also being explored. AIMs: Homology modeling of tyrosine kinase and Screening of compound library for tyrosine kinase inhibitors

• M.Sc. Bioinformatics Project: Pharmacophore Modeling in LEUKEMIA Disease

Abstract: Three genetically linked leukemic genes are selected from the chromosomes (ABL1 chro-9, AFF1 chro-4, MKL1 chro-22). From these genes we find out some interest Protein having more than four ligands, For Leukemia disease a best suitable Pharmacophore Model was prepared with the help of Ligandscout 1.03 Tool. If any drug can bind with this Pharmacophore then we can have 100% treatment of genetically linked LEUKEMIA. In this whole project study, we have refered to NCBI, TIGR, EBI, HPRD, and SWISS-PROT for data base.

• B.Sc. Project: Design of Fully Working Fermenter Model:

In this 2 litre fermenter model we can incubate Organism, add antifoaming agent, temperature control and have a couple of impeller to rotate. It uses 220 volt power supply. In a final Project of B.Sc. Microbiology.

• Attended workshop on “Biological sequences analysis and application in crop improvement” at G.B.Pant University (Uttarakhand) Nov16-17,2007
• Developed an Offline Web site For DOEACC final Project.
• Six month DOTNET training at Alien SoftNet Technologies Pvt. Ltd.

TECHNICAL SKILLS

AREA OF INTEREST

My area of interest is broad during my education, whatever I learned I applied in my work according to my needs. But I focused on Crime scene investigation, Fingerprint analysis, hair analysis, drugs analysis, paint analysis, fibre analysis, document analysis, foot print and blood pattern analysis. Software developer in DOTNET Technology. Computer Aided Drug Designing, Molecular Modelling, Computational Biology, Immunology, Recombinant DNA Technology, Genomics & Proteomics, Metabolic pathways, Gene Sequence Analysis, Protein Sequence Analysis, Discovery of genuine drugs for Cancer using Bioinformatics Tools, Phylogenetic Analysis, Virtual Screening and Docking.

PERSONAL PARTICULARS